Analytical Validation of BRAF Mutation Testing from Circulating Free DNA Using the Amplification Refractory Mutation Testing System
نویسندگان
چکیده
منابع مشابه
BRAF mutation testing in colorectal cancer.
Colorectal cancer is the second most common cause of cancer death in the United States. Understanding the biochemical pathways underlying carcinogenesis has paved the way for more effective treatments and better outcomes. BRAF mutation testing has a role in (1) differentiating sporadic colorectal cancer from Lynch syndrome, (2) identifying cancers lacking BRAF mutation that are more likely to r...
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The amplifi cation refractory mutation system (ARMS), which has also been described as allele-specifi c PCR (ASP) and PCR amplifi cation of specifi c alleles (PASA), is a PCR-based method of detecting single base mutations (Newton et al., 1989). ARMS has been applied successfully to the analysis of a wide range of polymorphisms, germline mutations and somatic mutations. The technique has the ab...
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We used a novel method based on allele-specific quantitative polymerase chain reaction (Intplex) for the analysis of circulating cell.free DNA (ccfDNA) to compare total ccfDNA and KRAS- or BRAF-mutated ccfDNA concentrations in blood samples from mice xenografted with the human SW620 colorectal cancer (CRC) cell line and from patients with CRC. Intplex enables single-copy detection of variant al...
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The idea of using models in mutation analysis dates back to mid 1980s. Recently, it is shown that, with some proper adaptations, mutation testing can also be utilized as a black-box testing approach. For this purpose, a modeling structure, a set of mutation operators, coverage criteria and test case generation methods are required. This paper compares event-based and state-based realizations of...
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ژورنال
عنوان ژورنال: The Journal of Molecular Diagnostics
سال: 2014
ISSN: 1525-1578
DOI: 10.1016/j.jmoldx.2013.12.004